HK-1-CAS9 cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

HK-1-CAS9 Order

Catalog#

YC-C095-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

HK-1-CAS9

Morphology

Epithelial-like,?adherent

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HK-1 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HK-1-CAS9 cell line that can maintain long-term, stable and high expression.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HK-1			STR Info (Cell bank) Cell Line:NPC/HK1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11.0	13.0
D13S317	11	11		11.0	11.0
D7S820	8	11		8.0	11.0
D16S539	9	11		9.0	11.0
VWA	18	19		18.0	19.0
TH01	7	9		7.0	9.0
AMEL	X	X
TPOX	8	11		8.0	11.0
CSF1PO	11	12		11.0	12.0

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
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High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
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